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Download AllBackground: Ascosphaera apis is a fungus belonging to the family Ascophaeraceae and affecting honeybee brood. This fungus causes the larval disease chalkbrood of honeybees (Apis mellifera). Larvae ingesting fungal spores that germinate within the digestive tract and compete with the larvae for food die of starvation. The fungus consumes the body of the larvae, resulting in a white and “chalky” appearance, hence the designation chalkbrood, and is most visible during wet springs with cooler outside temperatures. The chalky mummies are highly infectious and spores of this fungus often re-infect colonies. Ascosphaera apis causes large economic losses, particularly in combination with other pathogens.
Description: MycoReal Kit Ascosphaera apis uses real-time PCR for the detection of the the ITS1 region of Ascosphaera apis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEF00211 | 100 | FAM | VIC/HEX | ITS1 region |
| DVEF00213 | 100 | FAM | CY5 | ITS1 region |
| DVEF00251 | 50 | FAM | VIC/HEX | ITS1 region |
| DVEF00253 | 50 | FAM | CY5 | ITS1 region |
Shipping Temperature: +4°C cool packs
- Amplification and detection: ITS1 region of Ascosphaera apis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: MycoReal Kit Ascosphaera apis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV932 | 100 | FAM | - | ITS1 region |
Shipping Temperature: +4°C cool packs