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Product Manual BactoReal® Kit American Foulbrood 1.1 Download PDF
Material Safety Data Sheet BactoReal® Kit American Foulbrood 1.1 Download PDF
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Background:  American foulbrood (AFB) is a bacterial brood disease of honeybees caused by the Grampositive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Spores infect young bee larvae, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores, which may remain dormant for many years.

Description:  BactoReal® Kit American Foulbrood 1.1 uses real-time PCR for the detection of the 16S rRNA gene of Paenibacillus larvae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.

BactoReal® Kit American Foulbrood 1.1
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05413 100 FAM Cy5 16S rRNA
DVEB05453 50 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Paenibacillus larvae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit American Foulbrood 1.1 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Kit American Foulbrood 1.1
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV924 100 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.