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Product Manual BactoReal® Kit Aerococcus viridans Download PDF
Material Safety Data Sheet BactoReal® Kit Aerococcus viridans Download PDF
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Background:  Aerococcus viridans is a Grampositive bacterium causing gaffkemia, a fatal lobster disease, and it has been associated with septicemia in sea turtles. Aerococcus viridans has also been isolated from the milk of cows with subclinical mastitis. It can also cause different human infections.

Description:  BactoReal® Kit Aerococcus viridans uses real-time PCR for the detection of the 16S rRNA gene of Aerococcus viridans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Aerococcus viridans
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05313 100 FAM Cy5 16S rRNA
DVEB05353 50 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Aerococcus viridans
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Aerococcus viridans is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Aerococcus viridans
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV912 100 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.