Background:  Actinobacillus pleuropneumoniae (APP) causes respiratory disease in swine (pig haemorrhagic pleuropneumonia, actinobacillosis). It is a Gram-negative coccobacillus and a pathogen of the respiratory tract. Clinical signs of the acute disease are dyspnea, coughing, anorexia, depression, fever and sometimes vomiting. The disease can progress very rapidly and death can occur within a few hours. Chronic infections are characterized by cough and pleuritis. Many herds are infected with APP without presenting any clinical evidence of the disease. Carrier pigs harbour APP in their nasal cavities and/or tonsils.

Description:  BactoReal® Kit Actinobacillus pleuropneumoniae uses real-time PCR for the detection of the apxIVA gene of Actinobacillus pleuropneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Actinobacillus pleuropneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02013 100 FAM Cy5 apxIVA
DVEB02053 50 FAM Cy5 apxIVA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: apxIVA gene of Actinobacillus pleuropneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Actinobacillus pleuropneumoniae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Actinobacillus pleuropneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV550 100 FAM Cy5 apxIVA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.