Background:  Monkeypox is a smallpox-like disease caused by the monkeypox virus. Monkeypox virus belongs to the Orthopoxvirus genus. These viruses have a genome of double-stranded DNA and can replicate in the cytosol. The natural reservoir of monkeypox virus are rodents and can be transmitted from them to other animals and to humans. Further transmission from person to person can occur through contact with skin and bodily secretions. Monkeypox virus is endemic in Africa. Two genetically different clades have developed, the West African clade and the Congo Basin (Central Africa) clade. In May 2022 there was an increased incidence of monkeypox virus infections outside of Africa, especially in the US, Canada and Europe. This virus belongs to the West African clade.

Description:  ViroReal® Kit Monkeypox Virus is a real-time PCR test and detects the B9R gene of the monkeypox virus, which encodes the interferon-g receptor-like protein. The test was validated with a synthetic oligonucleotide representing this sequence.
A probe-specific amplification-curve in the FAM channel (530 nm) indicates amplification of monkeypox virus-specific DNA. As quality control, the kit contains an internal DNA positive control (DNA IPC), which is detected in the Cy5 channel. The target of the DNA IPC assay (artificial target DNA) can be extracted with the sample and serves as DNA extraction control as well as real-time PCR inhibition control. Ingenetix ViroReal® , BactoReal® , MycoReal® , PanReal and ParoReal® Kits are optimized to run under the same thermal cycling conditions. DNA and RNA can be analysed in one run.
An internal DNA positive control (DNA IPC) is detected in Cy5 channel and is used as DNA extraction as well as real-time PCR inhibition control. The target for the DNA IPC (artificial target DNA) is extracted with the sample.

ViroReal® Kit Monkeypox Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV02753 50 FAM Cy5 B9R
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: B9R Gen
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: This test has been validated with the QuantStudioTM 7 (Thermo Fisher Scientific) but is also suitable for use with other instruments such as ABI® 7500 Real-Time PCR System (Thermo Fisher Scientific), LightCycler® 480 II (Roche Diagnostics), Mx3005P® (Agilent), qTOWER3G (Analytik Jena), and mic instrument (bio molecular systems). Please, keep in mind that some PCR-platforms first have to be calibrated with the corresponding dye before performing multiplex-PCR.

BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.