Background:  Human metapneumovirus (hMPV) is an enveloped negative single-stranded RNA virus (ss (-)RNA) of the family Paramyxoviridae. It was isolated for the first time in 2001. HMPV is present as two genetic lineages, hMPV A and hMPV B. Human metapneumovirus causes upper and lower respiratory tract infections, symptoms very similar to respiratory syncytial virus (RSV). Human metapneumovirus has a worldwide distribution and it is one of the most common causes of lower respiratory infection in young children. Reinfections appear to be common. Co-infection of human metapneumovirus with influenza virus or RSV can occur.

Description:  ViroReal® Kit Metapneumovirus uses RT real-time PCR for the detection of the nucleocapsid protein gene of human metapneumovirus (hMPV) strains lineages hMPV A and hMPV B. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Metapneumovirus (IVD)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV01553 50 FAM Cy5 nucleocapsid protein gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: nucleocapsid protein gene of Metapneumovirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • CE-IVD Kit available in the following countries: AT, CY, FI, GR, IE, LU, MC, PT, SE, TR

PCR platforms: ViroReal® Kit Metapneumovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.