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Download All Background: The 16 species of Haemophilus are Gram-negative bacteria occasionally colonizing the mucosa of humans and animals. Some Haemophilus species are the causative agents of various infections. For example, H. aegyptius causes purulent conjunctivitis, H. parainfluenzae is a rare cause of a form of endocarditis. Haemophilus influenza can be found exclusively in the mucosa of humans, mainly in the upper respiratory tract. Transmission occurs through close contact with contaminated respiratory droplets. In sum, six capsular serotypes (a-f) can be distinguished, out of which type b (Hib) is the most prevalent and virulent one causing bloodstream infections and meningitis mainly in children less than 5 years. In contrast, unencapsulated strains (also called nontypeable H. influenzae, NTHi) of H. influenzae are part of the normal bacterial flora of humans and are occasionally pathogen. These strains can cause otitis, sinusitis, epiglottitis, bronchitis, pneumonia and less frequently meningitis and bacteraemia. The Omp P6 gene of H. influenzae is present in both the encapsulated and unencapsulated strains of H. influenzae.
Description: BactoReal® Kit Haemophilus influenzae uses real-time PCR for the detection of the Omp P6 gene of Haemophilus influenzae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB01053 | 50 | FAM | Cy5 | Omp P6 gene |
- Amplification and detection: Omp P6 gene of Haemophilus influenzae
- CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Haemophilus influenzae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.