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| Product Manual MycoReal® Kit Fungi |
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| Material Safety Data Sheet MycoReal® Kit Fungi |
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Background: Fungal diseases are mostly opportunistic infections with a great variety of common and uncommon pathogens. Up to 150 fungal species have been shown as potential human pathogens involving all body sites. Invasive mycoses are increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. These infections are mainly caused by yeasts (such as Candida spp., Cryptococcus neoformans, Saccharomyces cerevisiae, Trichosporon spp.) and moulds (such as Aspergillus spp., Scedosporium spp., Fusarium spp., Natrassia mangiferae, Curvularia spp., Schizophyllum commune, Paecilomyces variotii, Bipolaris spp., Cladophialophora bantiana, and zygomycetes such as Rhizopus spp., Absidia spp., Rhizomucor spp., Mucor spp., Cunninghamella bertholletiae, Saksenaea vasiformis, Apophysomyces elegans, Basidiobolus ranarum, Conidiobolus spp.). The mortality rate of invasive fungal infections is 40-100%. A rapid diagnosis improves the outcome. Non-invasive fungal infections (such as infections of the urogenitary tract, of the eye, of the skin, etc.) are mainly caused by Candida, Aspergillus, Acremonium, Fusarium and dermatophytes (such as Trichophyton spp., Microsporum spp.).
Description: MycoReal® Kit Fungi is a non-automated real-time PCR test for the qualitative universal (broad-range) detection and identification of DNA of fungi (ITS2-region). For phylogenetic identification of the pathogen, the PCR amplicon must be sequenced and analysed by BLAST. MycoReal® Kit Fungi does not displace culture but offers an improvement in the detection of fungi in cases where the presence of fungi is suspected but culture remains negative, or the fungus is difficult to cultivate. This kit is suitable for specimens taken from normally sterile sites. Proper specimens are DNA extracts isolated from samples of bronchoalveolar lavages (BAL), aspirates, cerebrospinal fluid, tissue, paraffin embedded tissue, fungal colonies as well as of environmental samples. The kit is suitable to a limited extent for the detection of fungi in blood samples. In case of a mixed fungal infection, the pathogen cannot be phylogenetically identified.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUF00453 | 50 | FAM | Cy5 | ITS2 |
- Amplification and detection: ITS2 region of fungi
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: MycoReal® KitFungi is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.