Background:  Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. The genus Borrelia consists of numerous species. Besides the species belonging to the B. burgdorferi sensu lato-complex there are also human pathogenic Borrelia species causing relapsing fever (B. recurrentis, B. duttonii and B. hermsii). Species belonging to the B. burgdorferi sensu lato-complex and causing Lyme disease (Lyme borreliosis) are Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae, B. bavariensis, B. bissettii and B. spielmanii. These are associated with different genospecies-specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description:  BactoReal® Kit Borrelia burgdorferi sensu lato uses real-time PCR for the detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato (s.l.) complex. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Borrelia burgdorferi sensu lato (IVD)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB05053 50 FAM Cy5 ospA gene
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ospA gene of Borrelia burgdorferi sensu lato
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Borrelia burgdorferi sensu lato is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche), Mx3005P® QPCR System (Agilent), CFX Opus Real-time PCR System (Bio-Rad), CFX Dx (Bio-Rad) and T-COR 8 (Tetracore), but is also suitable for other real-time PCR instruments.

BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.