Background: Aspergillus is a fungal genus consisting of several hundred species. Aspergillus species are ubiquitously found and some species can cause opportunistic infections such as allergic bronchopulmonary aspergillosis, pulmonary aspergilloma and invasive aspergillosis. Invasive aspergillosis is increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. Aspergillus fumigatus is the predominant disease-causing species. However, other species than A. fumigatus, in particular A. terreus and A. flavus but also A. niger, A. nidulans and A. ustus have gained greater clinical significance. Not only the detection, but also the identification of the fungal species is important. The species might provide information on a potential contamination, colonisation or infection and moreover antifungal resistance.
Description: MycoReal® Kit Aspergillus is a real-time PCR test for the detection of Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus niger and Aspergillus nidulans from samples purified from bronchoalveolar lavages (BAL), blood, aspirates, cerebrospinal fluid and tissue. . Furthermore, it detects some other closely related Aspergillus/Emericella species belonging to Aspergillus section Fumigati, Flavi, Terrei, Nigri, Nidulantes, Usti and Versicolori. The kit detects the internal transcribed spacer 2 (ITS2-region, multicopy gene) of Aspergillus. Due to the use of specific probes (FAM and VIC labelled) the test is highly specific for Aspergillus. It allows the detection and to some extent also the distinction of medically important Aspergillus species. Analysis is done with Assay Mix 1 and Assay Mix 2 in two parallel PCR-reactions. The internal DNA positive control (DNA IPC) is detected in Cy5 channel and is used as DNA extraction as well as PCR inhibition control. The DNA IPC target is extracted with the sample. Clinical Benefit MycoReal® Kit Aspergillus enables diagnosis of Aspergillus infections including invasive aspergillosis. The rapid Aspergillus detection and species discrimination without culture enables lifesaving early and targeted antifungal treatment of patients. Product features Highly specific and sensitive Detects Aspergillus group-specific ITS2 region. Multicopy targets for high test sensitivity. Analytical Specificity: 100% specificity for Aspergillus Analytical Sensitivity (LoD): LoD 95% between 2-11 target copies, depending on the species Clinical Sensitivity: 90.5%, including data for blood samples Analytical Specificity: 100% specificity for Aspergillus Analytical Sensitivity (LoD): LoD 95% between 2-11 target copies, depending on the species Clinical Sensitivity: 90.5% clinical sensitivity, including data for blood samples Detection of a wide range of Aspergillus species Detects the five clinically most relevant species A. fumigatus, A. flavus, A. terreus, A. niger and A. nidulans as well as some other closely related Aspergillus/Emericella species belonging to Aspergillus section Fumigati, Flavi, Terrei, Nigri, Nidulantes, Usti and Versicolori. Validated with blood samples Enables early diagnosis of invasive aspergillosis Discrimination of A. flavus, A. terreus and A. fumigatus Enables specific antifungal therapy
|FAM & VIC/HEX
Shipping Temperature: +4°C cool packs
- Amplification and detection: ITS2-region of Aspergillus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: MycoReal® Kit Aspergillus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific) and cobas z 480 Analyzer (Roche), but is also suitable for other real-time PCR instruments.