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Product Manual BactoReal® European Foulbrood | Download PDF |
Material Safety Data Sheet BactoReal® European Foulbrood | Download PDF |
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Background: European foulbrood (EFB) is a bacterial brood disease of honeybees caused by the Grampositive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae are infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Larvae that die from the disease have been starved of food.
Description: BactoReal® Kit European Foulbrood uses real-time PCR for the detection of the 16S rRNA gene of Melissococcus plutonius. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEB05513 | 100 | FAM | Cy5 | 16S rRNA |
DVEB05553 | 50 | FAM | Cy5 | 16S rRNA |
- Amplification and detection: 16S rRNA gene of Melissococcus plutonius
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit European Foulbrood is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV923 | 100 | FAM | Cy5 | 16S rRNA |