Back
Inquiry

Download

Product Manual BactoReal® Brucella spp. Download PDF
Material Safety Data Sheet BactoReal® Brucella spp. Download PDF
Download All

Background:  Brucellosis - also called Bang''s disease, Crimean fever, Gibraltar fever, Malta fever, Maltese fever, Mediterranean fever, rock fever, or undulant fever - is a zoonotic infection of domesticated and wild animals, caused by bacteria of the genus Brucella. Species infecting domestic livestock are B. melitensis (goats and sheep), B. suis (pigs), B. abortus (cattle and bison), B. ovis (sheep) and B. canis (dogs).

Description:  BactoReal® Kit Brucella spp. uses real-time PCR for the detection of the IS711 region of B. abortus, B. ovis, B. melitensis, B. suis and B. canis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brucella spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02113 100 FAM Cy5 IS711 region
DVEB02153 50 FAM Cy5 IS711 region
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS711 region of Brucella spp.
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Brucella spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brucella spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1201 100 FAM Cy5 IS711 region
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.