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| Product Manual MycoReal® Batrachochytrium dendrobatidis |
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| Material Safety Data Sheet MycoReal® Batrachochytrium dendrobatidis |
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Background: Batrachochytrium dendrobatidis, also known as the amphibian chytrid fungus, causes the disease chytridiomycosis in amphibians. It infects the keratinized skin of amphibians.
Description: MycoReal® Kit Batrachochytrium dendrobatidis uses real-time PCR for the detection of the the ITS2 region of Batrachochytrium dendrobatidis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEF00313 | 100 | FAM | Cy5 | ITS2 region |
| DVEF00353 | 50 | FAM | Cy5 | ITS2 region |
- Amplification and detection: ITS2 region of Batrachochytrium dendrobatidis
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: MycoReal® Kit Batrachochytrium dendrobatidis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV939 | 100 | FAM | Cy5 | ITS2 region |