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Download All Background: Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae, genus Circovirus and is an icosahedral, non-enveloped DNA virus with a single-stranded ambisense circular genome.
Description: ViroReal® Typing Kit PCV2 a, b, d contains three PCR primer sets for the amplification and detection of DNA of porcine circovirus type 2 (PCV2) a, b, d. This test is based on an intercalating dye using real-time PCR and has been developed and validated for the Applied Biosystems® 7500 (Fast) instrument (Thermo Fisher Scientific), but is also suitable for other real-time PCR instruments. This test allows the rapid typing of PCV2 a, b, d from DNA samples. ViroReal® TypingKit PCV2 a, b, d detects the capsid gene of PCV2 a,b, d. The test is based on a phylogenetic analysis and the definition of genotype-specific marker postions for PCV2 genotypes published by Franzo et al. The amplification curve and gene specific melting curve at 515nm indicate the amplification of PCV2 a, b, d specific DNA. Master mix and seperate positive controls for PCV2a, PCV2b and PCV2d are included.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVET002 | 100 | SYBR Green | capsid gene |
- Genotyping of porcine circovirus type 2 (PCV2): PCV2a, PCV2b, PCV2d
- Real-time PCR and melt curve analysis with supplied master mix
- ROX™ dye as passive reference
PCR platforms: ViroReal® Typing Kit PCV2a, b, d has been tested with Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.