Download
Product Manual BactoReal® Trueperella pyogenes |
|
Material Safety Data Sheet BactoReal® Trueperella pyogenes |
|
|
Background: Trueperella pyogenes (formerly Arcanobacterium pyogenes, Corynebacterium pyogenes, Actinomyces pyogenes) is a Grampositive commensal and opportunistic pathogen of economically important livestock, causing diseases such as mastitis, abscesses and pneumonia.
Description: BactoReal® Kit Trueperella pyogenes uses real-time PCR for the detection of the 16S rRNA gene of Trueperella pyogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEB05113 | 100 | FAM | Cy5 | 16S rRNA |
DVEB05153 | 50 | FAM | Cy5 | 16S rRNA |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: 16S rRNA gene of Trueperella pyogenes
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Trueperella pyogenes is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV910 | 100 | FAM | Cy5 | 16S rRNA |
Shipping Temperature: -20 °C to +4 °C