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Product Manual BactoReal® Streptococcus suis Download PDF
Material Safety Data Sheet BactoReal® Streptococcus suis Download PDF
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Background:  Streptococcus suis (S. suis) is a Grampositive, zoonotic bacterial pathogen of pigs and wild boars. It is associated with fever, sudden deaths, respiratory problems, and pneumonia, meningitis and fertility problems in pig herds, representing a substantial economic burden for the pig industry.

Description:  BactoReal® Kit Streptococcus suis uses real-time PCR for the detection of the fibrinogen binding protein (fbpS) gene of Streptococcus suis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus suis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06013 100 FAM Cy5 fbpS
DVEB06053 50 FAM Cy5 fbpS
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: fbpS gene of Streptococcus suis
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Streptococcus suis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Streptococcus suis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV233 100 FAM Cy5 fbpS
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects DNA of Streptococcus suis by targeting the fibrinogen binding protein (fbpS) gene using real-time PCR.
The assay is validated for DNA extracted from tissue samples, such as lung and tonsil.
The IPC is added during extraction and detected in the Cy5 channel to monitor DNA extraction quality and detect PCR inhibition.
The assay detects at least 1 DNA copy per reaction with a LoD95% of 25 copies and shows linearity from 100 to 1,000,000 copies.
The assay includes only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.