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Product Manual BactoReal® Streptococcus agalactiae Download PDF
Material Safety Data Sheet BactoReal® Streptococcus agalactiae Download PDF
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Background:  Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a beta-hemolytic Grampositive streptococcus. It is pathogenic primarily for animals, but can also infect humans. In cattle, it causes mastitis, which leads to diminished milk production. Transmission occurs during milking. Streptococcus agalactiae secretes a protein (CAMP factor) which causes haemolysis in the presence of beta-toxin of Staphylococcus aureus in blood agar.

Description:  BactoReal® Kit Streptococcus agalactiae uses real-time PCR for the detection of the cfb gene of S. agalactiae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus agalactiae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01513 100 FAM Cy5 cfb
DVEB01553 50 FAM Cy5 cfb
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: cfb gene of Streptococcus agalactiae
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Streptococcus agalactiae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Streptococcus agalactiae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV900 100 FAM Cy5 cfb
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Streptococcus agalactiae (Group B Streptococcus) by targeting the cfb gene using real-time PCR.
The assay is validated for DNA extracted from milk and similar veterinary materials.
An artificial DNA IPC added during extraction helps detect PCR inhibition and confirms extraction success.
The LoD95 is 3 copies per reaction, with a minimum sensitivity of 1 copy per PCR.
Assays include detection reagents only; kits also provide master mix, controls, and buffers.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.