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Product Manual ViroReal® PRRS Virus Type 1 & 2 Download PDF
Material Safety Data Sheet ViroReal® PRRS Virus Type 1 & 2 Download PDF
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Background:  The porcine reproductive and respiratory syndrome virus (PRRSV) is a single strain, positive-sense RNA virus belonging to the order Nidovirales, family Arteriviridae. PRRS is one of the most economically important diseases of swines and is also known as Blue-Ear Pig Disease. The disease is characterised by reproduction defects and by multifactor respiratory problems. European (EU) lineage PRRSV isolates (Betaarterivirus suid 1, PRRSV type 1; prototype strain is the lelystad virus) are genetically divergent from North American (US) isolates (Betaarterivirus suid 2, PRRSV type 2; prototype strain is the VR-2332), but cause similar clinical symptoms. A great genetic heterogeneity of field isolates is observed and further complicates diagnosis.

Description:  ViroReal® Kit PRRS Virus Type 1 & 2 uses one-step reverse transcription RT real-time PCR for the detection of the open-reading frame 7 including the 3'-untranslated region of both PRRSV lineages - Betaarterivirus suid 1 (PRRSV Type 1, EU) and Betaarterivirus suid 2 (PRRSV Type 2, NA). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit PRRS Virus Type 1 & 2
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV00113 100 FAM, VIC Cy5 open-reading frame 7
DVEV00153 50 FAM, VIC Cy5 open-reading frame 7
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: open-reading frame 7 of PRRSV Type 1 & 2
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms

PCR platforms: ViroReal® Kit PRRS Virus Type 1 & 2 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PRRS Virus Type 1 & 2
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1R01 100 FAM, VIC Cy5 open-reading frame 7
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects RNA of PRRSV Type 1 and Type 2 by targeting the ORF7 gene of each type.
The assay is validated for RNA extracted from blood, saliva, semen, feces, nasal secretions, milk, and tissue samples.
An artificial RNA internal positive control (IPC) is added during extraction and detected in the Cy5 channel to monitor RNA extraction efficiency and RT-PCR inhibition.
The assay detects 12 RNA copies per reaction for PRRSV EU and 20 RNA copies per reaction for PRRSV NA, with LoD95% values of 56 and 35 copies per reaction, respectively.
The assay contains only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.