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Product Manual BactoReal® Mycoplasma bovis Download PDF
Material Safety Data Sheet BactoReal® Mycoplasma bovis Download PDF
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Background:  Mycoplasma bovis is a pathogen causing respiratory disease, otitis media, arthritis and mastitis in cattle worldwide.

Description:  BactoReal® Kit Mycoplasma bovis uses real-time PCR for the detection of the rpoB gene of M. bovis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma bovis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02513 100 FAM Cy5 rpoB
DVEB02553 50 FAM Cy5 rpoB
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: rpoB gene of Mycoplasma bovis
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Mycoplasma bovis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma bovis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1300 100 FAM Cy5 rpoB
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects DNA of Mycoplasma bovis by targeting the rpoB gene.
The assay is validated for DNA extracted from synovial fluid, biopsies, bronchoalveolar lavage (BAL), swabs, and milk.
An artificial DNA internal positive control (IPC) is added during extraction and detected in the Cy5 channel to monitor extraction efficiency and PCR inhibition.
The assay has an analytical sensitivity of 2 target copies per PCR reaction, with a limit of detection (LoD95%) of 4 copies per reaction.
The assay contains only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.