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Product Manual ViroReal® PCV3 Download PDF
Material Safety Data Sheet ViroReal® PCV3 Download PDF
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Background:  Porcine circovirus type 3 (PCV3) is a novel circovirus species identified in pigs with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory disease and multi-systemic inflammation. Porcine circovirus is a small, non-enveloped DNA virus that contains a single strand of circular DNA. It belongs to the genus Circovirus in the family Circoviridae.

Description:  ViroReal® Kit PCV3 is a real-time PCR for the qualitative detection of the DNA of the capsid gene of porcine circovirus type 3 (PCV3). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit PCV3
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03613 100 FAM Cy5 capsid gene
DVEV03653 50 FAM Cy5 capsid gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: DNA of the capsid gene of PCV3
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PCV3 was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems) cobas z 480 Analyzer (Roche), and QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PCV3
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV103 100 FAM Cy5 capsid gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay is designed for the qualitative detection of porcine circovirus type 3 (PCV3) DNA using real-time PCR.
The assay targets a conserved region of the PCV3 capsid protein gene for specific detection.
The assay is validated for serum, blood, and tissue samples obtained from swine.
The primers and probe are designed to avoid cross-reactivity with PCV1, PCV2, or other porcine viruses.
The Assay includes only the primer/probe mix and a positive control, for labs with their own reagents. The Kit adds an internal control and ready-to-use RT-PCR reagents for a complete workflow.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.