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Product Manual ParoReal® Trichinella spiralis & britovi | Download PDF |
Material Safety Data Sheet ParoReal® Trichinella spiralis & britovi | Download PDF |
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Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide, four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.
Description: ParoReal® Kit Trichinella spiralis & britovi uses real-time PCR for the detection of the internal transcribed spacer of Trichinella spiralis and T. britovi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEP00413 | 100 | FAM, VIC | Cy5 | internal transcribed spacer |
DVEP00453 | 50 | FAM, VIC | Cy5 | internal transcribed spacer |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: internal transcribed spacer of Trichinella spiralis & T. britovi
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
PCR platforms: ParoReal® Kit Trichinella spiralis & britovi is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV268 | 100 | FAM, VIC | Cy5 | internal transcribed spacer |
Shipping Temperature: -20 °C to +4 °C