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Product Manual ParoReal® Kit Trichinella spiralis & britovi Download PDF
Material Safety Data Sheet ParoReal® Kit Trichinella spiralis & britovi Download PDF
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Background:  Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide, four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

Description:  ParoReal® Kit Trichinella spiralis & britovi uses real-time PCR for the detection of the internal transcribed spacer of Trichinella spiralis and T. britovi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Trichinella spiralis & britovi
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00413 100 FAM, VIC Cy5 internal transcribed spacer
DVEP00453 50 FAM, VIC Cy5 internal transcribed spacer
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: internal transcribed spacer of Trichinella spiralis & T. britovi
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Trichinella spiralis & britovi is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Trichinella spiralis & britovi
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV268 100 FAM, VIC Cy5 internal transcribed spacer
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.