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Product Manual ViroReal® PEDV & SDCV |
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Material Safety Data Sheet ViroReal® PEDV & SDCV |
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Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in swine of all ages. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).
Description: ViroReal® Kit PEDV & SDCV uses one-step reverse transcription multiplex RT real-time PCR for the detection of nucleocapsid protein gene (N gene) of PEDV (porcine epidemic diarrhea virus) and of the 3’UTR of SDCV (swine delta coronavirus, PDCoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV01913 | 100 | FAM, VIC | Cy5 | nucleocapsid protein gene |
DVEV01953 | 50 | FAM, VIC | Cy5 | nucleocapsid protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: N gene of PEDV and 3’UTR of SDCV
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
PCR platforms: ViroReal® Kit PEDV & SDCV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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RTGV69 | 100 | FAM, VIC | Cy5 | nucleocapsid protein gene |
Shipping Temperature: -20 °C to +4 °C