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| Product Manual BactoReal® Kit Mycoplasma hyopneumoniae |
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| Material Safety Data Sheet BactoReal® Kit Mycoplasma hyopneumoniae |
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Background: Mycoplasma hyopneumoniae is a primary pathogen of respiratory disease in pigs. This bacterium is one of the most important causative agents of the enzootic pneumonia (EP) and of the porcine respiratory disease complex (PRDC).
Description: BactoReal® Kit Mycoplasma hyopneumoniae uses real-time PCR for the detection of the rpoB gene of M. hyopneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB00113 | 100 | FAM | Cy5 | rpoB |
| DVEB00153 | 50 | FAM | Cy5 | rpoB |
- Amplification and detection: rpoB gene of Mycoplasma hyopneumoniae
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Mycoplasma hyopneumoniae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV210 | 100 | FAM | Cy5 | rpoB |