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Product Manual BactoReal® Kit Mycoplasma gallisepticum Download PDF
Material Safety Data Sheet BactoReal® Kit Mycoplasma gallisepticum Download PDF
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Background:  Avian mycoplasmosis is caused by several pathogenic mycoplasmae. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered the main species of pathogenic mycoplasmae. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

Description:  BactoReal® Kit Mycoplasma gallisepticum is a real-time PCR for the qualitative detection of the mgc2 gene (cytadhesin-like protein encoding gene) of M. gallisepticum. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma gallisepticum
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB00313 100 FAM Cy5 mgc2
DVEB00353 50 FAM Cy5 mgc2
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: mgc2 gene of Mycoplasma gallisepticum
  • Real-time PCR with rapid hot-start <i>Taq</i> DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma gallisepticum was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma gallisepticum
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV908 100 FAM Cy5 mgc2
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.