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Product Manual ViroReal® MERS-CoV Download PDF
Material Safety Data Sheet ViroReal® MERS-CoV Download PDF
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Background:  Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a beta coronavirus causing Middle East Respiratory Syndrome (MERS), a zoonotic and severe respiratory disease in humans. To date, MERS-CoV has only been isolated from dromedary camels and humans. In humans, it was first identified in April 2012. Camels are a likely primary source of the MERS-CoV infection in humans. Oro-nasal samples showed highest prevalence and younger animals show higher virus load.

Description:  ViroReal® Kit MERS-CoV is a real-time PCR for the qualitative detection of the RNA of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit MERS-CoV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03013 100 FAM Cy5 nucleocapsid (N) gene
DVEV03053 50 FAM Cy5 nucleocapsid (N) gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas z 480 Analyzer (Roche), and QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® MERS-CoV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV31 100 FAM Cy5 nucleocapsid (N) gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay detects RNA of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) by targeting the nucleocapsid (N) gene using real-time RT-PCR.
The assay is validated for RNA extracted from respiratory tract samples.
An artificial RNA IPC is added during extraction and detected in the Cy5 channel to monitor RNA extraction and detect PCR inhibition.
Highly specific primers and probes were selected, ensuring detection of all known MERS-CoV strains without cross-reactivity.
The assay includes detection reagents only, while the kit includes a full reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.