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Product Manual BactoReal® Kit Trueperella pyogenes Download PDF
Material Safety Data Sheet BactoReal® Kit Trueperella pyogenes Download PDF
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Background:  Trueperella pyogenes (formerly Arcanobacterium pyogenes, Corynebacterium pyogenes, Actinomyces pyogenes) is a Gram-positive commensal and opportunistic pathogen of economically important livestock, causing diseases such as mastitis, abscesses and pneumonia.

Description:  BactoReal® Kit Trueperella pyogenes is a real-time PCR for the qualitative detection of the 16S rRNA gene of Trueperella pyogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Trueperella pyogenes
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05113 100 FAM Cy5 16S rRNA
DVEB05153 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Trueperella pyogenes
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Trueperella pyogenes was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Trueperella pyogenes
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV910 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit qualitatively detects DNA of Trueperella pyogenes by targeting the 16S rRNA gene.
T. pyogenes is an opportunistic pathogen of livestock that can cause mastitis, abscesses, pneumonia, and other infections, leading to economic losses.
The internal positive control (IPC) monitors DNA extraction efficiency and detects potential PCR inhibition.
Validated sample materials include biopsies, bronchoalveolar lavage (BAL), swabs, and milk.
The detection limit is 10 target copies per PCR reaction.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.