Download
| Product Manual ViroReal® Kit Swine Influenza A (SIV) |
Download PDF
|
| Material Safety Data Sheet ViroReal® Kit Swine Influenza A (SIV) |
Download PDF
|
Download All
|
Background: Swine Influenza is an acute, febrile respiratory disease of swine with high morbidity and low mortality. In the industry it is commonly known as Swine Flu, Hog Flu, and Pig Flu. It is caused by the influenza virus, mainly influenza type A (H1N1), an enveloped virus with single-stranded, segmented RNA with negative polarity as genome. Influenza A virus infects a wide variety of warm-blooded animals including pigs, birds, horses as well as humans.
The infections in swine were initially due to transmission from infected humans, and then subsequently spread through pig-to-pig transmission. Pigs' ability to serve as a ‘mixing vessel’ depends on the presence of both human-adapted and avian-adapted receptor types in their respiratory tract. Further reassortment of previously circulating pig influenza viruses such as those of types A(H1N1), A(H1N2) and A(H3N2) with the, now officially designated A(H1N1)pdm09 influenza virus, is possible.
Description: ViroReal® Kit SIV is a real-time PCR for the qualitative detection of the of the matrix protein gene of the influenza A virus of warm-blooded animals including pigs, birds, horses as well as humans. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV00413 | 100 | FAM | Cy5 | matrix protein gene |
| DVEV00453 | 50 | FAM | Cy5 | matrix protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: matrix protein gene of Swine Influenza A
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit SIV was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena),MIC instrument (bio molecular systems), cobas Z 480 Analyzer (Roche)).
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV15 | 100 | FAM | Cy5 | matrix protein gene |
Shipping Temperature: -20 °C to +4 °C