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Product Manual ViroReal® Kit SDCV Swine Delta Coronavirus Download PDF
Material Safety Data Sheet ViroReal® Kit SDCV Swine Delta Coronavirus Download PDF
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Background:  Swine delta coronavirus (SDCV, also called porcine delta coronavirus PDCoV) is a member of the genus Coronavirus (Deltacoronavirus), family Coronaviridae, and a positive-sense, enveloped, single-stranded RNA virus. SDCV is a new virus first found in pigs in Hong Kong in 2012 and 2014 in the United States. Swine delta coronavirus causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. Swine delta coronavirus infection is clinically similar to, but genetically distinct from porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV).

Description:  ViroReal® Kit Swine Delta Coronavirus is a real-time PCR for the qualitative detection of the 3’UTR of swine delta coronavirus (PDCoV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit SDCV Swine Delta Coronavirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01813 100 FAM Cy5 3’UTR
DVEV01853 50 FAM Cy5 3’UTR
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: 3’UTR gene of Swine Delta Coronavirus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Swine Delta Coronavirus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas Z 480 Analyzer (Roche) QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® SDCV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV110 100 FAM Cy5 3’UTR
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The test targets the 3′ untranslated region (3′UTR) of Swine Delta Coronavirus RNA and uses a FAM-labeled probe for detection.
The IPC verifies the success of RNA extraction and helps detect reverse transcription or PCR inhibition, minimizing false-negative results.
Validated instruments include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, MIC, qTOWER3G, and cobas z 480.
Yes, the IPC can be added during extraction for full process control or to the PCR master mix to monitor the RT-PCR step only.
The test was validated against a range of viral strains and shows no cross-reactivity with PEDV, PRRSV, TGEV, HEV, PPV, or PCV2.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.