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Product Manual BactoReal® Kit Streptococcus spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Streptococcus spp. Download PDF
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Background:  Streptococcus is a genus of Grampositive bacteria belonging to the Firmicutes and the lactic acid bacteria group. There are currently over 50 species known in this genus. Many streptococcal species are nonpathogenic and form part of the commensal animal or human microbiome of the mouth, skin, intestine, and upper respiratory tract. Certain Streptococcus species are responsible for streptococcal pharyngitis, pink eye, meningitis, bacterial pneumonia, endocarditis, erysipelas, necrotizing fasciitis and bovine mastitis.

Description:  BactoReal® Kit Streptococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Streptococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03813 100 FAM Cy5 23S rRNA
DVEB03853 50 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Streptococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Streptococcus spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Streptococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV904 100 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 23S rRNA gene of Streptococcus spp. for specific detection.
Streptococcus species are associated with respiratory infections, endocarditis, skin and soft tissue infections, and mastitis in cattle.
The IPC confirms successful DNA extraction and excludes false negatives due to PCR inhibition.
Streptococcus DNA is detected in the FAM channel, and the IPC is detected in the Cy5 channel.
Primers and probes were validated for specificity; only very high concentrations of unrelated bacteria may cause weak cross-reactions.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.