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Product Manual BactoReal® Kit Staphylococcus spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Staphylococcus spp. Download PDF
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Background:  The genus Staphylococcus includes more than 50 different species. Staphylococci are commensals on skin and upper respiratory tracts of mammals and birds. However, they can also cause various infections of the skin and a wide variety of diseases in humans and animals through either toxin production or penetration. Another common path of infection is the respiratory tract, where the bacterium causes pneumonia. Staphylococci, mainly Staphylococcus aureus can also cause bovine mastitis.

Description:  BactoReal® Kit Staphylococcus spp. is a real-time PCR for the qualitative detection of the 23S rRNA gene of species of the genus Staphylococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Staphylococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03713 100 FAM Cy5 23S rRNA
DVEB03753 50 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Staphylococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Staphylococcus spp. was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Staphylococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV804 100 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 23S rRNA gene for specific detection of Staphylococcus DNA.
Staphylococci can cause skin infections, pneumonia, urinary tract infections, endocarditis, osteomyelitis, and bovine mastitis.
An internal positive control (IPC) is used to detect PCR inhibition and to confirm the quality of DNA extraction.
Staphylococcus DNA is detected in the FAM channel, and the IPC is detected in the Cy5 channel.
High concentrations of some non-Staphylococcus species may cause weak cross-reactions, but primers and probes are highly specific for Staphylococcus.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.