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| Product Manual ViroReal® Kit Rabies Virus |
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| Material Safety Data Sheet ViroReal® Kit Rabies Virus |
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Background: Rabies is caused by a neurotropic virus of the genus Lyssavirus of the family Rhabdoviridae, and is transmissible to all mammals. As it is transmissible to humans by inoculation or inhalation of infectious virus, all suspected infected material must be handled under the appropriate safety conditions specified by the World Health Organisation (WHO). Previous known genotypes were recently reclassified, and the Lyssavirus genus currently consists of 16 species (Aravan lyssavirus (ARAV), Australian bat lyssavirus (ABLV), Bokeloh bat lyssavirus (BBLV), Duvenhage lyssavirus (DUVV), European bat lyssavirus 1 (EBLV 1), European bat lyssavirus 2 (EBLV 2), Gannoruwa bat lyssavirus (GBLV), Ikoma lyssavirus (IKOV), Irkut lyssavirus (IRKV), Khujand lyssavirus (KHUV), Lagos bat lyssavirus (LBV), Lleida lyssavirus (LLEBV), Mokola lyssavirus (MOKV), Rabies lyssavirus (RABV), Shimoni bat lyssavirus (SHIBV) and the West Caucasian bat lyssavirus (WCBV). All lyssaviruses cause the severe neurotropic disease known as rabies. RABV (genotype 1, RABV-GT-1) is detected worldwide and is the prototype lyssavirus, the remaining species are known as rabies-related lyssaviruses. Human infections are mainly caused by RABV, DUVV, EBLV-1, EBLV-2 and ABLV.
ViroReal® Kit Rabies Virus detects the classical RABV genotype 1 and EBLV genotype 5 & 6. It does not detect LBV genotype 2, MOKV genotype 3, DUVV genotype 4 or ABLV genotype 7.
Description: ViroReal® Kit Rabies Virus is a real-time PCR for the qualitative detection of the nucleoprotein (N) gene of rabies virus. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV00813 | 100 | FAM | Cy5 | nucleoprotein gene |
| DVEV00853 | 50 | FAM | Cy5 | nucleoprotein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: N gene of Rabies Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit Rabies Virus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV255 | 100 | FAM | Cy5 | nucleoprotein gene |
Shipping Temperature: -20 °C to +4 °C