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Product Manual ViroReal® Kit RHDV |
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Material Safety Data Sheet ViroReal® Kit RHDV |
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Background: Rabbit haemorrhagic disease virus (RHDV), also called rabbit calicivirus (RCV) or european brown hare virus is a nonenveloped, positive-sense, single-stranded RNA calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD). This disease was first reported in China in 1984 and is a highly contagious and fatal disease for European rabbit (Oryctolagus cuniculus). There exist three distinct groups: the classic RHDV with the genogroups G1–G5 isolated from 1984 onwards, the antigenic variant RHDVa/G6 identified in 1996, and RHDV2 identified in 2010.
Description: ViroReal® Kit RHDV uses one-step reverse transcription RT real-time PCR for the detection of the capsid protein gene vp60 of rabbit haemorrhagic disease virus (RHDV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV03113 | 100 | FAM | Cy5 | vp60 |
DVEV03153 | 50 | FAM | Cy5 | vp60 |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: vp60 gene of RHDV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit RHDV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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RTGV32 | 100 | FAM | Cy5 | vp60 |
Shipping Temperature: -20 °C to +4 °C