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Product Manual BactoReal® Kit Pseudomonas aeruginosa Download PDF
Material Safety Data Sheet BactoReal® Kit Pseudomonas aeruginosa Download PDF
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Background:  Pseudomonas aeruginosa is a gram-negative bacterium found widely in the environment, such as in soil, water, and plants. It causes a wide range of opportunistic infections such as wound infections, mastitis, pneumoniae, otitis, etc. This pathogen causes acute and chronic infections and especially in immunocompromised patients bacteremia and Sepsis with high mortality rates. Because of the high intrinsic potential, severity of diseases and acquired resistance to many current antibiotics, it has been placed amongst the priority pathogens according to WHO (World Health Organization).

Description:  BactoReal® Kit Pseudomonas aeruginosa is a real-time PCR for the qualitative detection of the 16S rRNA gene of P. aeruginosa. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Pseudomonas aeruginosa
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB04513 100 FAM Cy5 16S rRNA
DVEB04553 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Pseudomonas aeruginosa
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Pseudomonas aeruginosa was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Pseudomonas aeruginosa
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV913 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 16S rRNA gene, with detection in the FAM fluorescence channel.
P. aeruginosa is a WHO-priority pathogen due to its intrinsic resistance and ability to cause acute and chronic infections in animals and humans.
P. aeruginosa DNA is detected in the FAM channel, while the IPC is detected in the Cy5 channel.
Positive and negative controls together with the IPC guarantee valid and reproducible results.
The kit is highly specific but may rarely cross-react with Pseudomonas protegens, P. otitidis, P. fluorescens, or P. tropicalis.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.