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Product Manual BactoReal® Kit Pseudomonas aeruginosa Download PDF
Material Safety Data Sheet BactoReal® Kit Pseudomonas aeruginosa Download PDF
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Background:  Pseudomonas aeruginosa is a Gram-negative bacterium found widely in the environment, such as in soil, water, and plants. It causes a wide range of opportunistic infections such as wound infections, mastitis, pneumoniae, otitis, etc.

Description:  BactoReal® Kit Pseudomonas aeruginosa uses real-time PCR for the detection of the 16S rRNA gene of P. aeruginosa. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Pseudomonas aeruginosa
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB04513 100 FAM Cy5 16S rRNA
DVEB04553 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Pseudomonas aeruginosa
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Pseudomonas aeruginosa is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Pseudomonas aeruginosa
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV913 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 16S rRNA gene, with detection in the FAM fluorescence channel.
P. aeruginosa is a WHO-priority pathogen due to its intrinsic resistance and ability to cause acute and chronic infections in animals and humans.
P. aeruginosa DNA is detected in the FAM channel, while the IPC is detected in the Cy5 channel.
Positive and negative controls together with the IPC guarantee valid and reproducible results.
The kit is highly specific but may rarely cross-react with Pseudomonas protegens, P. otitidis, P. fluorescens, or P. tropicalis.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.