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Product Manual ViroReal® Kit Porcine Parvovirus 1 |
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Material Safety Data Sheet ViroReal® Kit Porcine Parvovirus 1 |
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Background: Porcine parvovirus 1 (PPV 1) is a member of the Parvoviridae family with a single-stranded DNA as genome. PPV is associated with reproductive problems, including abortion, still births, small litters, neonatal deaths and weak piglets. PPV multiplies normally in the intestine of the pig without causing clinical signs. Disease occurs when sero-negative dams are infected in the first half of gestation and the virus crosses the placenta.
Description: ViroReal® Kit Porcine Parvovirus 1 uses real-time PCR for the detection of the non-structural protein 1 (NS1) gene of the porcine parvovirus 1 (PPV 1). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV00713 | 100 | FAM | Cy5 | non-structural protein 1 |
DVEV00753 | 50 | FAM | Cy5 | non-structural protein 1 |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: NS1 gene of Porcine Parvovirus 1
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit Porcine Parvovirus 1 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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RTGV010 | 100 | FAM | Cy5 | non-structural protein 1 |
Shipping Temperature: -20 °C to +4 °C