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Product Manual ViroReal® Kit PEDV (Porcine epidemic diarrhea) Download PDF
Material Safety Data Sheet ViroReal® Kit PEDV (Porcine epidemic diarrhea) Download PDF
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Background:  Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. If endemic, then diarrhea is observed with lower morbidity but higher mortality rate in suckling and recently weaned pigs. Infected sows and gilts show less reproduction performance. The PED virus is clinically similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).

Description:  ViroReal® Kit PEDV is a real-time PCR for the qualitative detection of the nucleocapsid protein gene (N gene) of porcine epidemic diarrhea virus (PEDV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit PEDV (Porcine epidemic diarrhea)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01313 100 FAM Cy5 nucleocapsid protein gene
DVEV01353 50 FAM Cy5 nucleocapsid protein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of PEDV
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PEDV was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PEDV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV80 100 FAM Cy5 nucleocapsid protein gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit targets the highly conserved nucleocapsid protein (N) gene of PEDV, detected in the FAM channel.
The RNA IPC helps verify RNA extraction and RT-PCR performance, reducing the chance of false-negative results.
Validated systems include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, MIC, qTOWER3G, and cobas z 480.
Primers and probes were validated in silico and experimentally; no cross-reactivity was observed with TGEV, PHEV, PRRSV, PPV, or PCV2.
If the IPC is not added during extraction, it can be added to the master mix to monitor only the RT-PCR reaction.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.