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Product Manual ViroReal® Kit PCV3 |
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Material Safety Data Sheet ViroReal® Kit PCV3 |
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Background: Porcine circovirus type 3 (PCV3) is a novel circovirus species identified in pigs with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory disease and multi-systemic inflammation. Porcine circovirus is a small, non-enveloped DNA virus that contains a single strand of circular DNA. It belongs to the genus Circovirus in the family Circoviridae.
Description: ViroReal® Kit PCV3 uses real-time PCR for the detection of capsid gene of the porcine circovirus type 3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV03613 | 100 | FAM | Cy5 | capsid gene |
DVEV03653 | 50 | FAM | Cy5 | capsid gene |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: capsid gene of PCV3
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit PCV3 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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RTGV103 | 100 | FAM | Cy5 | capsid gene |
Shipping Temperature: -20 °C to +4 °C