Download
Product Manual ViroReal® Kit PCMV |
|
Material Safety Data Sheet ViroReal® Kit PCMV |
|
|
Background: Porcine cytomegalovirus (PCMV) is a herpesvirus infecting only pigs. PCMV is present worldwide. Most PCMV infections are sub-clinical and clinical disease is rare causing inclusion body rhinitis (IBR), pneumonia, anaemia, fever and abortion or neonatal piglet losses. The virus may also be endemic without causing any apparent clinical disease or economic loss. However, these infected animals can be latent carriers of the virus.
Description: ViroReal® Kit PCMV uses real-time PCR for the detection of the DNA polymerase (DPOL) gene of the porcine cytomegalovirus (PCMV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEV01113 | 100 | FAM | Cy5 | DPOL |
DVEV01153 | 50 | FAM | Cy5 | DPOL |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: DPOL gene of PCMV
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit PCMV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV40 | 100 | FAM | Cy5 | DPOL |
Shipping Temperature: -20 °C to +4 °C