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Product Manual BactoReal® Kit Ornithobacterium rhinotracheale Download PDF
Material Safety Data Sheet BactoReal® Kit Ornithobacterium rhinotracheale Download PDF
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Background:  Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacterium causing respiratory disease in turkeys, chickens and other avian species. Clinical signs are nasal discharge, sneezing, coughing and sinusitis. Ornithobacterium rhinotracheale is currently the only species within the Ornithobacterium genus and found worldwide.

Description:  BactoReal® Kit Ornithobacterium rhinotracheale is a real-time PCR for the qualitative detection of the 16S rRNA gene of Ornithobacterium rhinotracheale. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Ornithobacterium rhinotracheale
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06613 100 FAM Cy5 16S rDNA
DVEB06653 50 FAM Cy5 16S rDNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Ornithobacterium rhinotracheale
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Ornithobacterium rhinotracheale was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Ornithobacterium rhinotracheale
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV927 100 FAM Cy5 16S rDNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects Ornithobacterium rhinotracheale DNA specifically through amplification of the 16S rRNA gene.
Infected poultry may show nasal discharge, sneezing, coughing, and sinusitis, often leading to severe respiratory disease.
Primers and probes were designed and validated to ensure high specificity for O. rhinotracheale and to avoid detection of unrelated bacteria.
An artificial DNA sequence serves as internal DNA positive control (IPC) to confirm DNA extraction success and rule out PCR inhibition.
The kit has been validated on ABI 7500, LightCycler 480 II, and Mx3005P, and is also compatible with instruments detecting FAM and Cy5 channels.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.