Background:  Nosema apis and Nosema ceranae are microsporidian parasites recently reclassified as a fungus. Both species cause nosema disease in honeybees (Apis mellifera). The dormant stages are long-lived spores. The most notable symptom is dysentery. Nosema ceranae can be detected in all four seasons, while Nosema apis occurs mostly in the milder seasons of autumn and spring. There is a higher mortality of bees when they are infected by Nosema ceranae than when infected with Nosema apis. Double infection is also possible.

Description:  MycoReal® Kit Nosema apis & ceranae is a multiplex real-time PCR for the qualitative detection and differentiation of the 18S rRNA gene of Nosema apis as well as of the 18S rRNA gene of Nosema ceranae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

MycoReal® Kit Nosema apis & ceranae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEF00113 100 FAM, VIC Cy5 18S rRNA gene
DVEF00153 50 FAM, VIC Cy5 18S rRNA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 18S rRNA of Nosema apis and 18S rRNA of Nosema ceranae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only
  • PCR platforms: MycoReal® Kit Nosema apis & Nosema ceranae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), LightCycler® 480 II (Roche Diagnostics) and Mx3005P® (Agilent). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

    Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

    MycoReal® Nosema apis & ceranae
    Order Number Reactions Channel Pathogen Channel IPC Target
    RTGV940 100 FAM, VIC Cy5 18S rRNA gene
    For veterinary use only
    Shipping Temperature: -20 °C to +4 °C
    BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.