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Product Manual BactoReal® Kit Mycoplasma suis Download PDF
Material Safety Data Sheet BactoReal® Kit Mycoplasma suis Download PDF
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Background:  Mycoplasma suis (formerly called Eperythrozoon suis) belongs to the hemotrophic mycoplasma group. It is the cause of swine eperythrozoonosis. Acute disease is associated with severe bacteremia and acute hemolytic anemia. In chronically infected pigs clinical signs range from mild icteroanemia, poor growth rates to bad reproductive performance.

Description:  BactoReal® Kit Mycoplasma suis is a real-time PCR for the qualitative detection of the 16S rDNA gene of M. suis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma suis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02613 100 FAM Cy5 16S rDNA
DVEB02653 50 FAM Cy5 16S rDNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rDNA gene of Mycoplasma suis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma suis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma suis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV215 100 FAM Cy5 16S rDNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects DNA of Mycoplasma suis by targeting the 16S rDNA gene using real-time PCR, providing reliable identification of the hemotrophic mycoplasma.
M. suis causes swine eperythrozoonosis, characterized by acute hemolytic anemia and bacteremia. Chronic infections may result in icteroanemia, reduced growth, and reproductive problems.
The kit is validated for DNA extracts from blood samples of swine.
The internal DNA positive control (IPC), added during extraction and detected in the Cy5 channel, ensures proper DNA extraction and identifies possible PCR inhibition.
The kit detects as little as 1 DNA copy per PCR reaction, with a LoD95% of 7 copies per reaction. No cross-reactions were observed with other tested Mycoplasma species, and M. parvum is not detected.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.