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Product Manual BactoReal® Kit Mycoplasma bovis Download PDF
Material Safety Data Sheet BactoReal® Kit Mycoplasma bovis Download PDF
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Background:  Mycoplasma bovis belongs to the class Mollicutes and is a cell wall-less bacterium that adheres to host cell surfaces. The organism is typically sensitive to tetracycline, although antimicrobial resistance has increaisngly been reported. The pathogen is causing respiratory disease, otitis media, arthritis, mastitis, keratoconjunctivitis, meningitis and endocarditis in cattle worldwide. It can affect all age groups and all sections.

Description:  BactoReal® Kit Mycoplasma bovis is a real-time PCR for the detection of the rpoB gene of M. bovis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma bovis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02513 100 FAM Cy5 rpoB
DVEB02553 50 FAM Cy5 rpoB
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: rpoB gene of Mycoplasma bovis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma bovis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma bovis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1300 100 FAM Cy5 rpoB
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects DNA of Mycoplasma bovis by amplifying the rpoB gene using real-time PCR with fluorescence measurement in the FAM channel.
M. bovis is linked to respiratory infections, mastitis, otitis media, arthritis, keratoconjunctivitis, meningitis, and endocarditis in cattle worldwide.
Validated materials include DNA extracts from synovial fluid, bronchoalveolar lavage (BAL), milk, swabs, and biopsies.
The internal DNA positive control (IPC), added during extraction and detected in the Cy5 channel, verifies DNA extraction efficiency and identifies possible PCR inhibition.
The kit detects as few as 2 target DNA copies per PCR reaction, with a LoD95% of 4 copies. No cross-reactions were observed with other tested Mycoplasma species.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.