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Product Manual BactoReal® Kit Mycobacterium avium ssp. paratuberculosis Download PDF
Material Safety Data Sheet BactoReal® Kit Mycobacterium avium ssp. paratuberculosis Download PDF
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Background:  Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne's disease, a chronic gastroenteritis affecting primarily ruminants and many other species. This disease is one of the most widespread and economically important diseases of ruminants. It has a relatively long incubation period, characterized by the excretion of MAP in faeces for months and years before first clinical symptoms develop.

Description:  BactoReal® Kit Mycobacterium avium ssp. paratuberculosis is a real-time PCR for the qualitative detection of the insertion sequence IS900 of M. avium ssp. paratuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycobacterium avium ssp. paratuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02213 100 FAM Cy5 IS900
DVEB02253 50 FAM Cy5 IS900
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS900 of Mycobacterium avium ssp. paratuberculosis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycobacterium avium ssp. paratuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV240 100 FAM Cy5 IS900
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit enables detection of Mycobacterium avium ssp. paratuberculosis (MAP) DNA by targeting the IS900 insertion sequence using real-time PCR, with fluorescence measured in the FAM channel.
MAP is the causative agent of Johne’s disease, a chronic gastroenteritis of ruminants that leads to long incubation, progressive weight loss, diarrhoea, and major economic losses in livestock farming.
Validated samples include DNA extracts from faeces, milk, and tissue materials from ruminants and other host species.
An internal DNA positive control (IPC), added during extraction and detected in the Cy5 channel, monitors extraction efficiency and identifies potential PCR inhibition.
The analytical sensitivity is 6 DNA copies per PCR with a LoD95% of 43 copies. Validation confirmed 100% specificity with no cross-reactions to other bacteria tested.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.