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Product Manual ParoReal® Kit Malpighamoeba mellificae Download PDF
Material Safety Data Sheet ParoReal® Kit Malpighamoeba mellificae Download PDF
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Background:  Malpighamoebia mellificae​ is related to the family of ​Amoebidae​ and effects, as a single-celled parasite, the excretory organs (malphigian tubules) of adult bees, but only of the western honeybee ​Apis mellifera​​. The intake cysts develop into trophozoites and damage the epithelia of the tubule. That causes disbalance between excretion and haemolymph exchange and leads to amoebiasis, that increases sudden mortality. Since the phase of excreting cysts is intermittent, or could even not happen during a longer period, there is a lack of microscopy detection. Often co-infections with Nosema are observed.

Description: 

​​ParoReal® Kit Malpighamoeba mellificae is a real-time PCR for the qualitative detection of the rRNA gene of Malpighamoeba mellificae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Malpighamoeba mellificae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00813 100 FAM Cy5 rRNA gene
DVEP00853 50 FAM Cy5 rRNA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: rRNA gene of Malpighamoeba mellificae 
  • Real-time PCR with rapid hot-start Taq DNA polymerase 
  • ROX™ dye as passive reference 
  • Internal Positive Control System to exclude false-negative results 
  • Optimized to handle PCR inhibitors 
  • PCR platforms: runs on all established standard real-time PCR platforms 
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ParoReal® Kit Malpighamoeba mellificae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit amplifies the rRNA gene of Malpighamoeba mellificae for specific detection.
It causes amoebiasis, leading to damage of the malpighian tubules, imbalance in excretion, and increased mortality in honey bee colonies.
The IPC confirms successful DNA extraction and identifies potential PCR inhibition, reducing the risk of false-negative results.
The kit has been validated on ABI 7500 and QuantStudio 5 and is compatible with other instruments detecting FAM and Cy5 channels.
Primers and probes were carefully designed and validated to detect Malpighamoeba mellificae specifically without cross-reactivity to Nosema or other pathogens.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.