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Product Manual ParoReal® Kit Malpighamoeba mellificae |
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Material Safety Data Sheet ParoReal® Kit Malpighamoeba mellificae |
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Background: Malpighamoebia mellificae is related to the family of Amoebidae and effects, as a single-celled parasite, the excretory organs (malphigian tubules) of adult bees, but only of the western honeybee Apis mellifera. The intake cysts develop into trophozoites and damage the epithelia of the tubule. That causes disbalance between excretion and haemolymph exchange and leads to amoebiasis, that increases sudden mortality. Since the phase of excreting cysts is intermittent, or could even not happen during a longer period, there is a lack of microscopy detection. Often co-infections with Nosema are observed.
Description:
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEP00813 | 100 | FAM | Cy5 | rRNA gene |
DVEP00853 | 50 | FAM | Cy5 | rRNA gene |
Shipping Temperature: -20 °C to +4 °C
PCR platforms: ParoReal® Kit Malpighamoeba mellificae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific) and was also tested with QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).