Background: 

Kashmir bee virus (KBV) is a positive single-stranded RNA virus within the family Dicistroviridae and the genus Aparavirus. It is closely related to Acute bee paralysis virus (ABPV) and to Israeli acute paralysis virus (IAPV), hence all three are summerized in the so-called AKI-complex. Members of that complex play a role in the sudden collapses of honeybee hives and colonies and cause economical losses worldwide. KBV infects adult bees, that die within days, and brood, but surviving larvae carry the virus latently. 

To distinguish between the Aparavirus species within the AKI-complex, ingenetix offers individual real-time PCR Kits for detection of Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus. Moreover a real-time PCR Kit for the universal detection of Aparaviruses is available.

Description: 

ViroReal® Kit Kashmir Bee Virus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of the Kashmir Bee Virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Kashmir Bee Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV04313 100 FAM Cy5
DVEV04353 50 FAM Cy5
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of capsid protein gene of Kashmer Bee Virus 
  • Real-time PCR with rapid hot-start Taq DNA polymerase 
  • ROX dye as passive reference 
  • Internal Positive Control System to exclude false-negative results 
  • Optimized to handle PCR inhibitors 
  • PCR- platforms: runs on all established standard real-time PCR- platforms 
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • PCR platforms:

    ViroReal® Kit Kashmer Bee Virus was validated with the QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) and the QuantStudio 7 Real-Time PCR System (Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

    BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.