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| Product Manual ViroReal® Kit Israeli Acute Paralysis Virus |
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| Material Safety Data Sheet ViroReal® Kit Israeli Acute Paralysis Virus |
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Background:
Israeli acute paralysis virus (IAPV) is a positive single-stranded RNA virus within the family Dicistroviridae and the genus Aparavirus. It is closely related to Acute bee paralysis virus (ABPV) and to Kashmir bee virus (KVB), hence all three are summarized in the so-called AKI-complex. That complex plays a role in the sudden collapses of honeybee hives and colonies, leading to severe economical losses worldwide. It causes systemic infections at all life-stages with extremely shaking wings and progressive paralysis as a nerval concomitant symptom. Infections increase during the season and the virus load peak is in winter. Therefore, weak colonies collapse in the early months of the year.
To distinguish between the Aparavirus species within the AKI-complex, ingenetix offers individual real-time PCR Kits for detection of Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus. Moreover a real-time PCR Kit for the universal detection of Aparaviruses is available.
Description:
ViroReal® Kit Israeli Acute Paralysis Virus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of Israeli acute paralysis virus. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
ViroReal® Kit Israeli Acute Paralysis Virus
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV04113 | 100 | FAM | Cy5 | capsid protein gene |
| DVEV04153 | 50 | FAM | Cy5 | capsid protein gene |
Shipping Temperature: -20 °C dry ice
Product Features:
- Amplification and detection: RNA of capsid protein gene of Israeli Acute Paralysis Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
PCR platforms:
ViroReal® Kit Israeli Acute Paralysis Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific), and was
also tested with QuantStudio™ 7 Real-Time PCR System (Thermo Fisher Scientific). It is also compatible with other
real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (e.g. ABI® 7500 Fast Real-Time PCR System, Mx3005P® (Agilent), QuantStudio™ 6 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems),
LightCycler® 480 II (Roche) und cobas z 480 Analyzer (Roche)).
Frequently Asked Questions:
The kit enables qualitative detection of RNA from Israeli Acute Paralysis Virus (IAPV) by targeting the capsid protein gene using real-time RT-PCR.
IAPV is part of the AKI complex and is associated with bee colony collapse. It causes systemic infections, paralysis, and shortened lifespan, especially during winter.
The kit includes an artificial RNA internal positive control (IPC) to monitor RNA extraction and detect RT-PCR inhibition. Detection occurs in the Cy5 channel.
The limit of detection (LoD95%) is approximately 27.6 RNA copies per reaction.
The kit is validated for RNA extracted from bee colony samples, including homogenized bee tissues.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.