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Product Manual ParoReal® Kit Histomonas meleagridis Download PDF
Material Safety Data Sheet ParoReal® Kit Histomonas meleagridis Download PDF
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Background:  Histomonas meleagridis is a parasitic protozoan causing blackhead disease, infectious enterohepatitis, or histomoniasis. It can infect turkeys, chickens, and occasionally other galliform birds but is most deadly in turkeys. Chickens are more resistant to the effects of the infection but may act as carriers. Histomonas meleagridis induces extensive and severe necrosis of the tissues of the mucosa and submucosa of cecum and parenchyma of the liver. It is transmitted by the nematode Heterakis gallinarum or directly from the droppings of infected birds. Histomonas meleagridis interacts with other gut organisms, such as bacteria and coccidia, and depends on them for full virulence.

Description:  ParoReal® Kit Histomonas meleagridis uses real-time PCR for the detection of the 18S rRNA gene of H. meleagridis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Histomonas meleagridis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00313 100 FAM Cy5 18S rRNA
DVEP00353 50 FAM Cy5 18S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 18S rRNA gene of Histomonas meleagridis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Histomonas meleagridis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Histomonas meleagridis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV267 100 FAM Cy5 18S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit enables the qualitative detection of Histomonas meleagridis DNA by targeting the 18S rRNA gene with real-time PCR.
Histomonas meleagridis causes blackhead disease (histomoniasis), a severe condition in turkeys and chickens leading to necrotic lesions in the cecum and liver.
The kit contains an artificial DNA internal positive control (IPC) to monitor DNA extraction efficiency and detect PCR inhibition.
The kit is validated for DNA extracted from biopsy samples of the cecum and liver.
The detection limit is approximately 10 DNA copies per PCR reaction, with a LoD95 of 12 copies per reaction.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.