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Product Manual BactoReal® Kit Enterococcus spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Enterococcus spp. Download PDF
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Background:  The genus Enterococcus includes more than 17 species and until 1984, Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Gram-positive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but also found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

Description:  BactoReal® Kit Enterococcus spp. is a real-time PCR for the qualitative detection of the 23S rRNA gene of species of the genus Enterococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Enterococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03413 100 FAM Cy5 23S rRNA
DVEB03453 50 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Enterococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Enterococcus spp. was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Enterococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV905 100 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects DNA of Enterococcus species by amplifying the 23S rRNA gene.
Enterococci are linked to diarrhea in calves, bovine mastitis, and opportunistic infections in humans and animals.
The IPC ensures the validity of results by monitoring extraction quality and excluding PCR inhibition.
The kit is designed for biopsies, blood, swabs, and milk samples.
The kit was validated against various bacterial species and showed no relevant cross-reactivity; weak signals may occur at very high concentrations of non-Enterococcus DNA.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.