Back
Inquiry

Download

Product Manual ParoReal® Kit Echinococcus multilocularis Download PDF
Material Safety Data Sheet ParoReal® Kit Echinococcus multilocularis Download PDF
Download All

Background:  Echinococcus multilocularis is a cyclophyllid tapeworm causing echinococcosis, a disease that affects many canid species including wolves, coyotes, foxes, jackals and domesticated dogs. Humans can also contract this disease. Echinococcus multilocularis requires two types of hosts to mature properly. It is transmitted to intermediate hosts via the ingestion of eggs in infected food. The eggs travel to the lungs, liver, and other internal organs where they develop into multilocular cysts. Finally, Echinococcus multilocularis is transmitted to definitive hosts by means of eating infected, cyst-containing organs. There it becomes attached to the tissues and grows.

Description:  ParoReal® Kit Echinococcus multilocularis is a real-time PCR for the qualitative detection of the  mitochondrial ND1 gene of E. multilocularis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00113 100 FAM Cy5 mitochondrial ND1
DVEP00153 50 FAM Cy5 mitochondrial ND1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ND1 gene of Echinococcus multilocularis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Echinococcus multilocularis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV266 100 FAM Cy5 mitochondrial ND1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit specifically amplifies the mitochondrial ND1 gene of E. multilocularis.
Intermediate hosts ingest eggs from contaminated food, leading to cyst formation in organs; definitive hosts are infected by consuming these cyst-containing tissues.
The IPC detects failures in DNA extraction or PCR inhibition, ensuring accurate interpretation of results.
The kit is validated for ABI 7500, LightCycler 480 II, and Mx3005P, and compatible with other instruments detecting FAM and Cy5 channels.
Primers and probes were designed and validated to detect only E. multilocularis without cross-reactivity to related species.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.